细胞类型:SV40转化细胞
是否是肿瘤细胞:0
物种来源:人
ATCC Number:CRL-1584?
数量:大量
生长状态:贴壁生长
器官来源:胎盘
运输方式:冻存运输
规格:15*20cm Designations: 3A-sub E [post crisis of 3A(tPA-30-1)]
Depositors: ?MI Cour
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Organ: placenta
Cell Type: SV40 transformed
Cellular Products:at 40C the cells produce human chorionic gonadotropin (hCG)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 11,13
D7S820: 10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,17
Comments:This line was established from ATCC CRL-1583 cells that had begun to senesce.
By passage 20 the cells had recovered and appeared capable of unlimited proliferation.
The saturation density (6 X 10 exp5 cells/sq cm) is increased and the morphology is altered relative to the parental line.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, rinse culture with fresh 0.25% trypsin-0.03% EDTA solution and let sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020