年限:32 years
ATCC Number:CCL-114?
生长状态:悬浮生长
数量:大量
器官来源:外周血
运输方式:冻存运输
细胞形态:淋巴样
细胞类型:B淋巴细胞
是否是肿瘤细胞:0
物种来源:人
规格:1mg Designations: RPMI 7666 [Gerner 7666]
Depositors: ?GE Moore
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphoblast;
Cellular Products:immunoglobulin; Epstein-Barr virus (EBV)
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Antigen Expression:HLA A3, Aw29, B7, B12; Blood type B+
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,11
D5S818: 11,12
D7S820: 8,11
THO1: 7,9.3
TPOX: 8,11
vWA: 14,16
Age: 32 years
Gender: male
Ethnicity: Caucasian
Comments:Biological Safety CLASS II.
EBNA positive.
The cells produce Epstein-Barr virus.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 5 X 10 exp5 viable cells/ml.
References: 22312: Moore GE, et al. Culture of normal human leukocytes. JAMA 199: 519-524, 1967. PubMed: 4960081