细胞类型:B淋巴细胞
ATCC Number:CRL-1805
相关疾病:淋巴瘤是否是肿瘤细胞:0
物种来源:猕猴
生长状态:悬浮生长
运输方式:冻存运输
年限:5 years
数量:大量
细胞形态:
淋巴样
Designations: LCL 8664
Depositors:
Rangan
Biosafety Level 生物安全等级 :2 [Cells Contain HERPESVIRUS ]
Shipped 提供形式 :frozen
Medium Serum: See Propagation
Growth
Properties:suspension
Organism:Macaca mulatta
Morphology:lymphoblast
Source:
Disease: lymphoma
Cell Type: B lymphocyte;
Cellular Products:B lymphotropic herpesvirus
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Age:5 years
Gender:male
Comments 注释 :CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard. Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.
About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen.
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium,Catalog No. 30-2001.
To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:Subcultivation
Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 4 X 10 exp5 cells/ml and maintain between 4 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References 参考文献 : 22860: Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macacamulatta) with malignant lymphoma.Int. J. Cancer 38: 425-432,1986. PubMed: 3017870