细胞形态:成纤维样
数量:大量
ATCC Number:CCL-197?
相关疾病:正常
品系:C57BL/10
组织来源:skeletal muscle
细胞类型:成纤维细胞
运输方式:冻存运输
是否是肿瘤细胞:0
物种来源:小鼠
生长状态:贴壁生长
年限:30 days
规格:0.1ml Designations: NOR-10
Depositors: ?BC Starcher
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Tissue: skeletal muscle
Strain: C57BL/10
Disease: normal
Cell Type: fibroblast
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Antigen Expression:H-2b
Age: 30 days
Comments:This may be used as a matched control for ATCC CCL-198 (BLO-1).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.1% trypsin for about 10 minutes, dislodge cells and centrifuge to remove from trypsin. Add fresh medium to cell pellet, resuspend cells in fresh medium and dispense into new flasks. Subculture every 3 to 5 days.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%