生长状态:悬浮生长
细胞形态:淋巴样
免疫类型:IgG1
是否是肿瘤细胞:0
物种来源:小鼠
ATCC Number:HB-9579?
运输方式:冻存运输
数量:大量
规格:0.5ml Designations: S6F1
Depositors: ?Dana Farber Cancer Inst.
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against a subset of human CD8 (LFA-1) positive T cells
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Comments:Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody recognizes a subset of CD8 positive T cells that have cytotoxic effector activity.
The S6F1 epitope is absent on CD8 positive suppressor cells.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 0.1 mM hypoxanthine, and 0.032 mM thymidine, 80%; heat-inactivated fetal bovine serum, 10%; heat-inactivated horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21964: Schlossman SF, Morimoto C. Monoclonal antibody specific to a novel epitope of the LFA-1 antigen of human T lymphocytes. US Patent 5,002,869 dated Mar 26 1991