生长状态:混合型生长
品系:C57BL
细胞形态:其他
是否是肿瘤细胞:0
物种来源:小鼠
ATCC Number:CRL-1642?
运输方式:冻存运输
数量:大量
器官来源:肺
相关疾病:其他疾病
规格:0.5mg
Designations 统一别名 : LL/2 (LLC1)
Depositors:JS Bertram
Biosafety Level 生物安全等级 :1
Shipped:frozen
Medium & Serum: See Propagation
Growth Properties:mixed, adherent and suspension
Organism:Mus musculus
Morphology:rounded - loosely attached or floating
Source:
Strain: C57BL
Organ: lung
Disease: Lewis lung carcinoma
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Applications 用途 :Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.
Tested and found negative for ectromelia virus (mousepox).
The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.
This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.
Tumorigenic:Yes
Antigen
Expression:H-2b
Comments 注释 :This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.
Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.
The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.
The cells are reported to be highly tumorigenic, but weakly metastatic in mice.
The cells form multilayers in flasks without actually becoming confluent.
Tested and found negative for ectromelia virus (mousepox).
Propagation:ATCC complete growth medium:The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:fetal bovine serum to a final concentration of 10%.
Temperature 培养温度 : 37.0°C
Subculturing:
Protocol: Subcultures are prepared by diluting the suspension 1:4 to 1:6.
Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature 保藏温度 : liquid nitrogen vapor phase
Doubling Time: 21 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References 参考文献 :1091: Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139
45212: Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function.J. Immunol. 163: 5020-5028, 1999. PubMed:10528207