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平台编号：bio-136764
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细胞信息： Human TLR9 Overexpressing - THP-1 Reporter Monocytes
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规格：3-7 x 10e6 cells
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用途：invivnogen原装
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注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用（产品信息以出库为准）
Human TLR9 Overexpressing - THP-1 Reporter Monocytes
Dual reporter monocytes for TLR9 pathway studies
INVIVOGEN ALSO OFFERS:
• HEK-Blue
hTLR9 cells
• HEK-Blue mTLR9 cells
• TLR9 ligands - Including stimulatory and inhibitory ODNs, as well as bacterial DNAInvivoGen offers a human monocyte-derived cell line, specifically designed for the study of human TLR9 (Toll-Like Receptor 9) signaling pathways:• THP1-Dual hTLR9 cellsTHP1-Dual hTLR9 cells overexpress the human TLR9 (hTLR9) gene. They also feature two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase) activities, respectively.
Unlike their parental cells, THP1-Dual, which weakly express TLR9, THP1-Dual hTLR9 cells are responsive to stimulation with TLR9 agonists, such as oligonucleotides containing CpG motifs (CpG ODNs). As expected, hTLR9 overexpression in THP1-Dual cells allows potent NF-κB and IRF responses upon incubation with CpG-ODNs of class A (ODN 2216), class B (ODN 2006, ODN 1826), and class C (ODN 2395). Of note, THP1-Dual hTLR9 cells are more responsive to the class B, human-preferred, ODN 2006 (see Figures). Background:
Toll-Like Receptor 9 (TLR9) is one of the most studied pattern recognition receptors (PRRs) for nucleic acids. It is an endosomal receptor that triggers NF-κB- and IRF-mediated pro-inflammatory responses upon the recognition of unmethylated cytosine-phosphorothioate-guanosine (CpG) forms of DNA [1-3]. TLR9 agonists can be mimicked by synthetic oligonucleotides containing CpG motifs (CpG ODNs) [1,3].
More detailsKey features:
Verified overexpression of TLR9 gene by qRT-PCR
Functionally validated with a selection of TLR9 agonists
Readily assessable Lucia luciferase and SEAP reporter activities
Applications:
Study of IRF and NF-κB-dependent TLR9 signaling pathways
Screening of novel specific activators or inhibitors of the TLR9 signaling pathways
References1. Kumagai Y. et al., 2008. TLR9 as a key receptor of the recognition of DNA. Adv. Drug. Deliv. Rev. 60(7):795-804.
2. Heinz L.X. et al., 2021. TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9. Nature. 581(7808):316-322.
3. Kayraklioglu N. et al., 2021. CpG oligonucleotides as vaccine adjuvants. DNA Vaccines: Methods and Protocols. Methods in Molecular Biology. Vol. 2197. p51-77.
• HEK-Blue
hTLR9 cells• HEK-Blue
mTLR9 cells• TLR9 ligands - Including stimulatory and inhibitory ODNs, as well as bacterial DNAInvivoGen offers a human monocyte-derived cell line, specifically designed for the study of human TLR9 (Toll-Like Receptor 9) signaling pathways:• THP1-Dual
hTLR9 cellsTHP1-Dual hTLR9 cells overexpress the human TLR9 (hTLR9) gene. They also feature two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase) activities, respectively.Unlike their parental cells, THP1-Dual
, which weakly express TLR9, THP1-Dual hTLR9 cells are responsive to stimulation with TLR9 agonists, such as oligonucleotides containing CpG motifs (CpG ODNs). As expected, hTLR9 overexpression in THP1-Dual cells allows potent NF-κB and IRF responses upon incubation with CpG-ODNs of class A (ODN 2216), class B (ODN 2006, ODN 1826), and class C (ODN 2395). Of note, THP1-Dual hTLR9 cells are more responsive to the class B, human-preferred, ODN 2006 (see Figures). Background:Toll-Like Receptor 9 (TLR9) is one of the most studied pattern recognition receptors (PRRs) for nucleic acids. It is an endosomal receptor that triggers NF-κB- and IRF-mediated pro-inflammatory responses upon the recognition of unmethylated cytosine-phosphorothioate-guanosine (CpG) forms of DNA [1-3]. TLR9 agonists can be mimicked by synthetic oligonucleotides containing CpG motifs (CpG ODNs) [1,3].
Verified overexpression of TLR9 gene by qRT-PCR
Functionally validated with a selection of TLR9 agonists
Readily assessable Lucia luciferase and SEAP reporter activities
Applications:
Study of IRF and NF-κB-dependent TLR9 signaling pathways
Screening of novel specific activators or inhibitors of the TLR9 signaling pathways
References1. Kumagai Y. et al., 2008. TLR9 as a key receptor of the recognition of DNA. Adv. Drug. Deliv. Rev. 60(7):795-804.
2. Heinz L.X. et al., 2021. TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9. Nature. 581(7808):316-322.
3. Kayraklioglu N. et al., 2021. CpG oligonucleotides as vaccine adjuvants. DNA Vaccines: Methods and Protocols. Methods in Molecular Biology. Vol. 2197. p51-77.
