pWH1266
(ATCC® 77092™)
Designations pWH1266
Depositors W Hillen
Biosafety Level 1
Vector Information
Size (kb): 8.89
Vector: pWH1266 (plasmid)
Construction: pBR322, pWH1277
Marker(s):ampR,tetR
Construct size (kb): 8.89
Features: marker(s): ampR, tetR
Replicon: pMB1, pWH1277
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.8; PvuII--4.4 (doublet); AccI--3.8, 2.9, 1.2, 0.9; HincII--4.6, 3.1, 1.1; EcoRI--8.8.
Contains the following restriction sites (approximate kb from the EcoRI site): BamHI--0.38; PvuII--2.1, 6.6; AccI--0.65, 4.5, 5.7, 6.7; HincII--0.65, 3.7, 8.4.
The 2.2 AccI/HincII fragment is essential for replication in A. calcoaceticus.
Ampicillin and tetracycline resistance are both expressed in A. calcoaceticus.
Shuttle vector.
The cryptic plasmid pWH1277 isolated from A. calcoaceticus lwoffi was linearized with PvuII and cloned into the PvuII site of pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References
Hunger M, et al. Analysis and nucleotide sequence of an origin of DNA replication in Acinetobacter calcoaceticus and its use for Escherichia coli shuttle plasmids. Gene 87: 45-51, 1990. PubMed: 2185139
Shipped frozen
Shipping Information plasmid in E. coli