Human herpesvirus 3 病毒
(ATCC? VR-1367?) 编号
Classification Herpesviridae, Varicellovirus
Deposited As Varicella-Zoster Agent Human herpesvirus 3
Strain 描述 Ellen
Common Name 普通名称 Varicella zoster virus
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions Liquid nitrogen vapor phase
Comments 注释 VR-1367? is a mycoplasma-free item derived at ATCC? from VR-586? by three passages in the presence of a mycoplasma removal agent. Effect on Host CPE, syncytial rounding and sloughing
Recommended Host MRC-5 (ATCC? CCL-171?)
Growth Conditions 生长条件
Temperature 培养温度 : 36℃
Recommendations For Infection:
This product is produced by co-cultivation of virus with fresh host cells. Seed culture vessels at 1 x 105 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.01) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 36℃ in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation. Incubation: 4-7 days
Effect on Host
CPE, syncytial rounding and sloughing Name of Depositor EI Rosanoff, Wyeth Laboratories, Inc.
Special Collection NCRR
Contract Source Vesicular fluid from child with chickenpox,
Georgia. Derived from VR-586?.
Treated to remove Mycoplasma hominis at ATCC?.
References Brunell PA, Casey HL. Crude tissue culture antigen for determination of Varicella-Zoster complement fixing antibody. Public Health Rep. 79: 839-842, 1964. PubMed: 14213793
Brunell PA. Transmission of chickenpox in a school setting prior to the observed exanthem. Am. J. Dis. Child. 143: 1451-1452, 1989. PubMed: 2556025