Encephalomyocarditis virus(Cell culture adapted)拉丁名
(ATCC® VR-1762™)菌株编号
Classification分类: Picornaviridae, Cardiovirus /
Product Format提供形式: frozen
Classification Picornaviridae, Cardiovirus Agent Encephalomyocarditis virus (Cell culture adapted)
Biosafety Level生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format提供形式 frozen
Storage Conditions保藏方法 -70℃ or colder
Comments注释 This isolate was deposited to ATCC after five passages in weanling mice and two passages in suckling mice. It was adapted to growth in cell culture by three passages in Vero cells (ATCC® CCL-81™).
Effect on Host CPE in the form or refractile rounding and sloughing Recommended Host Vero cells (ATCC® CCL-81™)
Growth Conditions生长条件
Temperature培养温度: 37℃
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and rinse cell sheet with 1X DPBS.
Remove 1X DPBS and then inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.01. Adsorb for 1 hour at 37℃ in a humidified 5% CO2 atmosphere.
End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 1 to 4 days at 37℃ in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture. Effect on Host CPE in the form or refractile rounding and sloughing
