Monkey cytomegalovirus
(ATCC® VR-677™)
Classification: Herpesviridae, Cytomegalovirus /
Product Format: frozen
Agent Monkey cytomegalovirus Strain 68-1
Common Name African green monkey cytomegaloviru
Applications Produces enlarged, distorted nuclei with bizarre inclusions infected human and monkey
fibroblast TC. Has morphology of the herpes group (by EM).
Biosafety Level 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation Urine of rhesus monkey, 1968
Product Format frozen
Storage Conditions -70℃ or colder (or colder)
Comments Sensitive to heat (exposure to 56C for 10 min. completely inactivated 10(4) TCID[50] of Rhesus CMV). Produces enlarged, distorted nuclei with bizarre inclusions infected human and monkey fibroblast TC. Has morphology of the herpes group (by EM). E
ffect on Host Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing) CPE, refractile rounding and sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2% Recommended
Host Recommended Host: MRC-5 (ATCC CCL-171)
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 35.0℃ Duration: 3 to 30 days;
For best results, cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent) at inoculation.
Cryopreservation Storage temperature: -70℃
Effect on Host Yes, in vitro effects:
Cytopathic effects (refractile rounding and sloughing) CPE, refractile rounding and sloughing