Human herpesvirus 5
(ATCC? VR-1780?)
Classification: Herpesviridae, Cytomegalovirus /
Product Format: frozen 1.0 mL per vial
Agent Human herpesvirus 5
Strain ATCC-2011-3
Common Name Human cytomegalovirus
Biosafety Level 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen 1.0 mL per vial
Storage Conditions Vapor phase of liquid nitrogen Comments Produced by co-cultivation with fresh host cells
Effect on Host CPE, refractile rounding
Recommended Host MRC-5 cells (ATCC? CCL-171?)
Growth Conditions
Temperature: 37℃
Recommendations for Infection:
This product is produced by co-cultivation of virus with fresh host cells.
Prepare a bulk cell suspension on the day of inoculation. Seed culture vessels at 1.3-1.5 x 104 cells per cm2.
Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1 – 1) and then dilute virus in the virus growth medium to prepare the virus inoculum.
Add virus inoculum to culture vessels.
Incubate for 24 hours at 37℃ in a humidified 5% CO2
atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then added fresh virus growth medium to cultures.
Continue incubation.
Incubation: 5 - 7 days at 37℃ in a humidified 5% CO2 atmosphere, until CPE has progressed through 80% of the monolayer.
Effect on Host CPE, refractile rounding
Name of Depositor ATCC Source
Clinical patient positive for CMV, Virginia, July 2011