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NCA 26 嗜热脂肪土芽孢杆菌

NCA 26 嗜热脂肪土芽孢杆菌

  • 平台编号:bio-72725
  • 拉丁属名:Geobacillus stearothermophilus
  • 规格:freeze-dried
  • 注意事项:仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用
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Geobacillus stearothermophilus (Donk) Nazina et al. 拉丁名

(ATCC® 12980™) 统一编号

Deposited As Bacillus stearothermophilus Donk

Strain Designations 菌株别名 NCA 26

Application 用途 Produces restriction endonuclease BstPI; Steam sterilization control; Sterility assurance; Pharmaceutical and Personal Care;

Biosafety Level 生物安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏条件

Frozen 冷冻物: -80℃ or colder

Freeze-Dried 冻干物: 2℃ to 8℃

Live Culture 活物: See Propagation Section

Preceptrol® yes

Type Strain 模式菌株 yes

Medium 培养基 ATCC® Medium 3: Nutrient agar or nutrient broth

Growth Conditions 生长条件

Temperature 培养温度: 55℃

Atmosphere 需氧情况: Aerobic

Name of Depositor 寄存人 Nat. Canners Assn.

References 参考文献. J. Microbiol. 6: 557, 1960

. J. Gen. Microbiol. 34: 270, 1964. Pugatsch T, Weber H. A thermostable, sequence-specific restriction endonuclease from Bacillus stearothermophilus: BstPI. Nucleic Acids Res. 7: 1429-1444, 1979. PubMed: 503858

U.S. Pharmacopeia USP Monographs: Biological Indicator for Steam Sterilization, Paper Carrier. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Nazina TN, et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and .... Int. J. Syst. Evol. Microbiol. 51: 433-446, 2001. PubMed: 11321089

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

Terminal Sterilization and Sterilization Indicators. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.15.

Cross References Nucleotide (GenBank) : U34874 Bacillus stearothermophilus levansucrase (surB) gene, complete cds. Nucleotide (GenBank) : X93600 B.stearothermophilus aspC gene.

Nucleotide (GenBank) : M57457 B.stearothermophilus alpha amylase gene, complete cds.

Nucleotide (GenBank) : U34875 Bacillus stearothermophilus levanase (surC) gene, complete cds.

Nucleotide (GenBank) : M63307 Bacillus stearothermophilus histidine utilization operon (hut) gene sequence.

Nucleotide (GenBank) : M26646 B.stearothermophilus manganese superoxide dismutase (MnSOD) gene, complete cds.

Nucleotide (GenBank) : AF055578 Bacillus stearothermophilus surface layer protein precursor (sbsC) gene, complete cds.

Nucleotide (GenBank) : AF139535 Bacillus stearothermophilus trp RNA-binding attenuation protein (mtrB) gene, complete cds.

Nucleotide (GenBank) : AF162268 Bacillus stearothermophilus insertion sequence ISBst12 putative transposase gene, complete cds.

Nucleotide (GenBank) : AF139534 Bacillus stearothermophilus chorismate mutase (aroH), anthranilate synthase subunit (trpE), and anthranilate phophoribosyl transferase (trpD) genes, complete cds.

安瓿瓶冻干管打管说明
 
一、打管说明
1、安瓿瓶开封:用浸过75%酒精的脱脂棉擦净安瓿管,用火焰加热其顶端,滴少量无菌水至加热顶端使之破裂,用锉刀或者镊子敲下已破裂的安瓿管顶端。
2、菌株恢复培养:用无菌吸管吸取0.3--0.5ml适宜的液体培养基,滴入安瓿管内,轻轻振荡,使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液,移植于1-2支建议的培养基试管中,并在建议的条件下培养。
3、注意事项:菌种活化前,请将安瓿管保存在6-10℃的环境下,某些菌种经过冷冻干燥保存后,延迟期较长,需要连续两次继代培养才能正常生长。
4、复苏后的菌种在传1-2代后使用。
5、暂不启开的安瓿及复苏后需保藏的斜面应于4℃中保藏。
特别注意事项
l、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时问过长会导致菌种衰退;
2、菌种操作应在无菌条件下进行,防止杂菌污染:
3、斜面菌种保藏时间通常为1-2个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为5~10年;
4、菌种使用过程中如出现杂菌污染或菌种生产性能下降,应及时与中国微生物菌种查询网联系或更换新的菌种。

  西林瓶和甘油冻存管打管说明
 
二、西林瓶菌种打管复活操作步骤
1、用 75%酒精棉擦拭西林瓶表面进行消毒。 
2、在生物安全柜中打开铝制瓶盖和胶盖。 
3、用无菌吸管吸取 0.5ml 左右液体培养基(《菌种说明书》中固体培养基去掉琼脂即可)于西 林瓶中将冻干菌粉全部溶解。 
4、将溶解后的菌悬液转移至盛有 4~5mL 液体培养基的试管中混匀,可将残留在吸管中的 1-2 滴菌悬液转接至固体培养基上。
5、将液体试管和斜面试管于推荐条件下静置培养,以液体培养结果为准。
三、甘油冻存管菌种打管复活操作步骤 
1、准备好推荐的菌种培养基(见《菌种说明书》)。
2、将冻存管下半部浸入 37℃温水中轻轻摇动,使冷冻状态的菌种悬液迅速融化。如果收到菌 种冻存管时菌悬液是融化状态则需要立即进行转接培养。 3、用 75%酒精棉擦拭冻存管表面进行消毒。
4、在生物安全柜中打开冻存管瓶盖,用无菌吸管吸取全部菌悬液,均匀涂布到 2 支试管斜面 或平板上。 
5、将斜面试管或平板于推荐条件下静置培养。对于生长缓慢的菌种应持续培养 2 周至更长的 时间。
四、注意事项 
1、 所有打管操作都需要无菌操作。需由专业微生物技术人员在相应防护设备中进行,生物危 害程度为三类的菌种应在生物安全柜中操作。 
2、 西林瓶和冻存管打开后需一次用完,不能留存。 
3、 菌种经过冻干保藏后,处于休眠状态,需要转接 2-3 代恢复活力。
4、 大部分冻干菌种会在培养几天后生长。有些菌种第一代恢复生长时会有较长的延迟期,培养此类菌种时需要将培养时间延长至正常培养时间的双倍时长,甚至 2 周至更长的时间。