Geobacillus stearothermophilus (Donk) Nazina et al. 拉丁名
(ATCC 7953) 统一编号
Deposited As
Bacillus stearothermophilus Donk
Application 用途 Gas sterilization control
Steam sterilization control
Sterility assurance
Sterilization control
Pharmaceutical and Personal Care
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏方法
Frozen 冻干物 : -80℃ or colder
Freeze-Dried 冷冻物 : 2℃ to 8℃
Live Culture 活菌 : See Propagation Section yes
Type Strain 模式菌株 no
Comments 注释 This strain is recommended by ATCC for use in the tests described in military specification MIL S-36586A and U.S. Pharmacopeia, 23rd rev.,, pp. 1976-1981, 1995 where only the taxon is specified.
Stated to grow at 35℃
The ATCC lab was unable to verify growth at 35℃.
Medium 培养基 ATCC Medium 3: Nutrient agar or nutrient broth
Growth Conditions 生长条件
Temperature 培养温度 : 55℃
Atmosphere 需氧情况 : Aerobic
Name of Depositor 寄存人 CP Hegarty
References 参考文献 Smith NR, et al. Aerobic spore forming bacteria. US Dep Agric Monogr 16: 1-148, 1952.
Arch. Biochem. Biophys. 54: 154-161, 1955.
British Pharmacopoeia Commission Methods of sterilization. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVIII, 2003
European Pharmacopoeia Commission Biological indicators of sterilization. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.2, 1997
U.S.
Pharmacopeia USP Monographs: Biological Indicator for Steam Sterilization, Paper Carrier. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011
Terminal Sterilization and Sterilization Indicators. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.15.
Sterilization of health care products--Biological indicators--Part 3: Biological indicators for moist heat sterilization.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11138-3:1995.
Sterilization of health care products --- Biological indicators, Part 3: Biological indicators for moist heat sterilization processes. London, UK:British Standards Institution;British Standard BS EN ISO 11138-3:2006.
Sterilization of health care products --- Biological indicators, Part 5: Biological indicators for low-temperature steam and formaldehyde sterilization processes. London, UK:British Standards Institution;British Standard BS EN ISO 11138-5:2006.
Cross References
Nucleotide (GenBank) : L13418 Bacillus stearothermophilus maltose permease (malA) gene, complete cds.
Nucleotide (GenBank) : M16814 B.stearothermophilus small acid-soluble spore protein (B-type SASP) gene, complete cds.
Nucleotide (GenBank) : M86508 Bacillus stearothermophilus proton glutamate symport protein (gltP) gene, complete cds.
Nucleotide (GenBank) : M13061 B.stearothermophilus SASP-1 gene encoding a small acid-soluble spore protein, complete cds.
Nucleotide (GenBank) : AF184067 Bacillus stearothermophilus MetS (metS) gene, partial cds; transition state regulator (abrB) gene, complete cds; and yabC pseudogene, partial sequence.
Nucleotide (GenBank) : U18943 Bacillus stearothermophilus mannitol transport protein (MtlA), putative transcriptional regulator (MtlR), mannitol enzyme IIA (MtlF) and mannitol-1-phosphate dehydrogenase (MtlD) genes, complete cds.
