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金黄色葡萄球菌

金黄色葡萄球菌

  • 平台编号:bio-53868
  • 拉丁属名:Staphylococcus aureus subsp. aureus Rosenbach
  • 规格:freeze-dried
  • 注意事项:仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用
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Staphylococcus aureus subsp. aureus Rosenbach 拉丁名

(ATCC® 25923™) 统一编号

Strain Designations 菌种别名 : Seattle 1945 /

Type Strain 模式菌株: no /

Biosafety Level 生物安全等级 : 2

Deposited As Staphylococcus aureus Rosenbach Strain Designations Seattle 1945

Application CAMP test

Assay of wood smoke concentrate

Control strain

Control strain for identification

Evaluation of Mueller-Hinton agar

Examination of dairy products

Media testing Quality control strain

Reference material

Susceptibility disc testing

Enteric Research

Water testing

Susceptibility disc testing ampicillin

Susceptibility disc testing caryomycin

Susceptibility disc testing cephalexin

Susceptibility disc testing cephaloglycin

Susceptibility disc testing cephaloridine cephalomycin S

usceptibility disc testing cephalothin

Susceptibility disc testing chloramphenicol

Susceptibility disc testing erythromycin

Susceptibility disc testing gentamicins gentamicin

Susceptibility disc testing kanamycin

Susceptibility disc testing novobiocin

Susceptibility disc testing tetracycline

Susceptibility testing CAMP test for Listeria monocytogenes

Quality control strain for Abbott, API, and Autobac products

Isolation 分离基物 Clinical isolate

Type Strain 模式菌株  no

Biosafety Level 生物安全等级  2

Product Format 提供形式  freeze-dried

Storage Conditions 保藏方法

Frozen(冷冻物): -80℃ or colder

Freeze-Dried(冻干物): 2℃ to 8℃

Live Culture(活物): See Propagation Section

Preceptrol® yes

Type Strain 模式菌株 no

Comments 注释   Incorrectly cited in previous editions of this catalog as identical to NCTC 6571.

Medium培养基  ATCC® Medium 18: Trypticase Soy Agar/Broth

Growth Conditions 生长条件

Temperature 培养温度: 37℃

Atmosphere 需氧情况: Aerobic

Name of Depositor 寄存人  FDA

Chain of Custody 来源国家 ATCC <-- FDA <-- F Schoenknecht

Isolation 分离基物 Clinical isolate

References 参考文献   Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8. Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A. Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M26-A.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3. U.S. Food & Drug AdministrationListeria monocytogenes, CAMP testIn: U.S. Food & Drug AdministrationBacteriological analytical manual8th ed.Gaithersburg, MDAOAC Internationalp. 10.09, 1995

US General Services Administration Mueller Hinton Medium, dehydrated. Washington, DC:US General Services Administration;Commercial Item Description A-A-50904A, 1986

. . WHO Expert Committee on Biological Standardization, 28th Report, WHO Tech. Rep. Ser. 610: 122-123, 1977

. . . Literature issued December 20, 1970 on Cephalothin Discs by Eli Lilly and Co., Indianapolis, IN. : . Fretheim K, et al. Influence of generation temperature on the chemical composition, antioxidative, and antimicrobial effects of wood smoke. J. Food Sci. 45: 999-1002, 1980.

Boyle VJ, et al. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3: 418-424, 1973. PubMed: 4790600

Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

API RAPIDEC Staph. bioMerieux. BD Phoenix Gram Positive AST PMIC/ID QC Set. BD Becton Dickinson; Phoenix Abbreviated Identification of Bacteria and Yeast; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M35-A2. Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A.

Detection and Enumeration of Listeria monocytogenes in Foods, the CAMP Test. Washington, DC:US Food & Drug Administration;Bacteriological Analytical Manual Online Chapt. 10.F, 2003 Identification of L. monocytogenes colonies from isolation agars. Washington, DC:American Public Health Association;APHA APHA2001-36.52, 2001

Food microbiology. Method 2.15: Examination for specific organisms--Listeria monocytogenes in dairy products. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 1766.2.15:1998.

Food microbiology. Method 2.16.1:Examination for specific organisms--Food and animal feeding stuffs--Horizontal method for the detection and enumeration of Listeria monocytogenes- Detection method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 1766.2.16.1:1998. Food microbiology. Method 5: Preparation of culture media, diluents and reagents. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 1766.5-1994.

Water microbiology. Method2: Culture media, diluents and reagents. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.2-1995.

Water microbiology. Method 8: Faecal streptococci--Estimation of the most probable number(MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.8-1995. Water microbiology. Method 9: Faecal streptococci -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.9-1995.

Food microbiology. Method 12.3: Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of coagulase-positive staphylococci (Staphyloccus aureus and other species)--Detection and MPN technique for low numbers. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 5013.12.3:2004.

Methods for Antimicrobial Dilution and Disk susceptibility Testing of Infrequently Isolated or Fastidious Bacteria: Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M45-A.

Microbiology of food and animal feeding stuffs-- Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)--Part3: Detection and MPN technique for low numbers. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 6888-3:2003.

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Microbiology of food and animal feeding stuffs--Horizontal method for the detection and enumeration of Listeria monocytogenes--Part 1: Detection method. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11290-1:1996.

Microbiology of food and animal feeding stuffs--Horizontal method for the detection and enumeration of Listeria monocytogenes-- Part 2: Enumeration method. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11290-2:1998.

Applied Biosystems, MicroSeq Food Panel. Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-3: 1989

Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-7: 1994, 1994

Medical microbiology: methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents; determination of MICs by agar dilution method. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-6: 1989, 1989

Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003

Medical microbiology--Culture media -- Part 4: Transport systems for specimens containing bacteria. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-4: 2003, 2003

Method 5.040: Listeria monocytogenes cultural method. Washington, DC:American Public Health Association;Standard Methods for the Examination of Dairy Products, 17th edition 2004. Microbiology of food and animal feeding stuffs --- Horizontal method for the detection and enumeration of Listeria monocytogenes --- Part 1: Detection method, Annex B [BS 5763-18:1997]. London, UK:British Standards Institution;British Standard BS EN ISO 11290-1:1997.

Microbiology of food and animal feeding stuffs --- Horizontal method for the detection and enumeration of Listeria monocytogenes --- Part 2: Enumeration method, Annex B. London, UK:British Standards Institution;British Standard BS EN ISO 11290-2:1998.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

BD GeneOhm MRSA ACP. BD Becton Dickinson. BD GeneOhm StaphSR. BD Becton Dickinson. ISO/CD 11133:2009,

Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions) Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM. BD MAX™ MRSA Assay. BD Becton Dickinson.

Cross References

Nucleotide (GenBank) : AX110511 Sequence 1244 from Patent WO0123604.

Nucleotide (GenBank) : AX110995 Sequence 1728 from Patent WO0123604.

Nucleotide (GenBank) : Z16422 S.aureus dfrB gene for dihydrofolate reductase.

Nucleotide (GenBank) : U39769 Staphylococcus aureus 16S-23S ribosomal RNA spacer region.

Nucleotide (GenBank) : U02910 Staphylococcus aureus ATCC 25923 16S rRNA gene, partial sequence.

Nucleotide (GenBank) : AF053568 Staphylococcus aureus ATCC 25923 heat shock protein 60 gene, partial cds.

Nucleotide (GenBank) : AB047239 Staphylococcus aureus DNA, complete structure of cassette chromosome(SCC)-like element, strain:ATCC25923.

安瓿瓶冻干管打管说明
 
一、打管说明
1、安瓿瓶开封:用浸过75%酒精的脱脂棉擦净安瓿管,用火焰加热其顶端,滴少量无菌水至加热顶端使之破裂,用锉刀或者镊子敲下已破裂的安瓿管顶端。
2、菌株恢复培养:用无菌吸管吸取0.3--0.5ml适宜的液体培养基,滴入安瓿管内,轻轻振荡,使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液,移植于1-2支建议的培养基试管中,并在建议的条件下培养。
3、注意事项:菌种活化前,请将安瓿管保存在6-10℃的环境下,某些菌种经过冷冻干燥保存后,延迟期较长,需要连续两次继代培养才能正常生长。
4、复苏后的菌种在传1-2代后使用。
5、暂不启开的安瓿及复苏后需保藏的斜面应于4℃中保藏。
特别注意事项
l、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时问过长会导致菌种衰退;
2、菌种操作应在无菌条件下进行,防止杂菌污染:
3、斜面菌种保藏时间通常为1-2个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为5~10年;
4、菌种使用过程中如出现杂菌污染或菌种生产性能下降,应及时与中国微生物菌种查询网联系或更换新的菌种。

  西林瓶和甘油冻存管打管说明
 
二、西林瓶菌种打管复活操作步骤
1、用 75%酒精棉擦拭西林瓶表面进行消毒。 
2、在生物安全柜中打开铝制瓶盖和胶盖。 
3、用无菌吸管吸取 0.5ml 左右液体培养基(《菌种说明书》中固体培养基去掉琼脂即可)于西 林瓶中将冻干菌粉全部溶解。 
4、将溶解后的菌悬液转移至盛有 4~5mL 液体培养基的试管中混匀,可将残留在吸管中的 1-2 滴菌悬液转接至固体培养基上。
5、将液体试管和斜面试管于推荐条件下静置培养,以液体培养结果为准。
三、甘油冻存管菌种打管复活操作步骤 
1、准备好推荐的菌种培养基(见《菌种说明书》)。
2、将冻存管下半部浸入 37℃温水中轻轻摇动,使冷冻状态的菌种悬液迅速融化。如果收到菌 种冻存管时菌悬液是融化状态则需要立即进行转接培养。 3、用 75%酒精棉擦拭冻存管表面进行消毒。
4、在生物安全柜中打开冻存管瓶盖,用无菌吸管吸取全部菌悬液,均匀涂布到 2 支试管斜面 或平板上。 
5、将斜面试管或平板于推荐条件下静置培养。对于生长缓慢的菌种应持续培养 2 周至更长的 时间。
四、注意事项 
1、 所有打管操作都需要无菌操作。需由专业微生物技术人员在相应防护设备中进行,生物危 害程度为三类的菌种应在生物安全柜中操作。 
2、 西林瓶和冻存管打开后需一次用完,不能留存。 
3、 菌种经过冻干保藏后,处于休眠状态,需要转接 2-3 代恢复活力。
4、 大部分冻干菌种会在培养几天后生长。有些菌种第一代恢复生长时会有较长的延迟期,培养此类菌种时需要将培养时间延长至正常培养时间的双倍时长,甚至 2 周至更长的时间。