ATCC® Number:VR-538™
Classification: Herpesviridae, Cytomegalovirus
Agent: Human herpesvirus 5 deposited as Cytomegalovirus
Strain: AD-169
Original Source: Tonsillectomy-adenoidectomy, 7-year-old girl
Depositors: WA Chappell
Biosafety Level:2
Shipped: frozen
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Incubation : Temperature: 36.0℃ Duration: 3-11 days, 5% CO2 in air atmosphere is recommended
Effect : Yes, in vitro effects: Cytopathic effects (cell rounding and detachment) in tissue culture
Comments : Stable for 6 months at -60C in the form of glycerinated cells. Virus primarily cell associated. Passage best effected by co-infection of fresh cells with infected cells. A long (10-11 day) incubation is required for initial passage of frozen stocks. Add contents of ampule to a 90-95% monolayer of HDF cells. If foci of CPE fail to spread by day 11, split infected cells 1:2 and observe for CPE as monolayer reforms. Strain AD-169 has been completely sequenced. Genbank: X17403
Related Products: recommended serum:ATCC 30-2020Virus DNA: ATCC VR-538D
References : 4079: ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002). 21416: Huang L, et al. The variable e' endds of a human cytomegalovirus oriLyt transcript (SRT) overlap an essential, conserved replicator element. J. Virol. 70: 5272-5281, 1996. PubMed: 8764037 21463: ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002). 30419: Noraz N, et al. Human cytomegalovirus-associated immunosuppression is mediated through interferon-alpha. Blood 89: 2443-2452, 1997. PubMed: 9116288 32520: Prichard MN, et al. Human cytomegalovirus uracil DNA glycosylase is required for the normal temporal regulation of both DNA synthesis and viral replication. J. Virol. 70: 3018-3025, 1996. PubMed: 8627778 32751: Iskenderian AC, et al. Four of eleven loci required for transient complementation of human cytomegalovirus DNA replication cooperate to activate expression of replication genes. J. Virol. 70: 383-392, 1996. PubMed: 8523552 33630: Rowe WP, et al. Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids. Proc. Soc. Exp. Biol. Med. 92: 418-424, 1956. PubMed: 13350367