Human herpesvirus 1 拉丁名
(ATCC® VR-733™) 统一编号
Classification Herpesviridae, Simplexvirus
Deposited As Herpes simplex virus 1
Agent Human herpesvirus 1
Strain F
Common Name Herpes simplex virus 1
Applications 用途 Susceptibility testing Testing virucides
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 1 mL per vial
Storage Conditions 保存方法 -70°C or colder
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims.
Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments 注释 Deposited as Herpes simplex virus 1.
This strain exhibits slight cross reactivity with certain Human herpesvirus 2 monoclonals.
Effect on Host CPE, refractile rounding and cell clumping
Recommended Host HEp-2 (ATCC® CCL-23™)
Growth Conditions 生长条件
Temperature 培养温度 : 34°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent.
Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1).
Adsorb 1-2 hours at 34°C in a humidified 5% CO2 atmosphere while rocking continuously.
End adsorption by adding virus growth medium.
Incubation: 1-3 days
Effect on Host CPE, refractile rounding and cell clumping
Name of Depositor 寄存人 B Roizman, Institut Merieux
U.S. Patent 4,760,079
U.S. Patent Number 4,760,079
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims.
Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Special Collection NCRR Contract
Source Facial vesicle
References 参考文献 Antiviral Susceptibility Testing: Herpes Simplex Virus by Plaque Reduction Assay;
Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M33-A.
ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension.
West Conshohocken, PA (Reapproved 2002).
ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).
Ejercito PM, et al. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J. Gen. Virol. 2: 357-364, 1968. PubMed: 4300104
Frankel N, Roizman B. Herpes simplex virus: genome size and redundancy studied by renaturation kinetics. J. Virol. 8: 591-593, 1971. PubMed: 4331657
Kieff ED, et al. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J. Virol. 8: 125-132, 1971. PubMed: 4329966
Kieff ED, et al. Genetic relatedness of type 1 and type 2 herpes simplex viruses. J. Virol. 9: 738-745, 1972. PubMed: 4337161
Roizman B. Perspectives in Virology VIII. New York, NY: Academic Press; .