ATCC® Number:VR-2093™
Classification: Picornaviridae, Hepatovirus
Agent: Hepatitis A virus deposited as Hepatitis A
Strain: HM 175 (uncloned)
Original Source: Human stool specimens from outbreak of virus, Australia
Depositors: NIH
Biosafety Level:2
Shipped: frozen
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Host Organism : Cercopithecus aethiops
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 21 to 28 days; For best results cells should be 24 to 48 hours old and 60-80% confluent (not 100% confluent).
Effect : Yes, in vitro effects: Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate
Store at : -70°C (or colder)
References : 3568: Feinstone SM, et al. Isolation of hepatitis A virus strain HM-175. US Patent 4,532,215 dated Jul 30 1985 4079: ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002). 21463: ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002). 32846: Daemer RJ, et al. Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect. Immun. 32: 383-393, 1981. PubMed: 6260685