ATCC® Number:VR-1378™
Classification: Paramyxoviridae, Paramyxovirinae, Rubulavirus
Agent: Human parainfluenza virus 4a deposited as Parainfluenza 4a
Strain: M-25
Original Source: Throat swab of patient with mild upper respiratory tract illness, 1958
Depositors: JC Hierholzer
Biosafety Level:2
Shipped: frozen
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Host Organism : LLC-MK2 cells (ATCCCCL-7.1) primary monkey kidney; human embryonic kidney cells; hamster embryonic kidney cells; bovine embryonic kidney cells; NCI-H292 cells (ATCCCRL-1848); LLC-MK2 cells (ATCCCCL-7)
Incubation : Duration: 7 days at 36C with rocking
Effect : Yes, in vitro effects: Cytopathic effects (variable) Yes, in vitro effects: hemadsorption and hemagglutination of guinea pig RBC
Comments : Same strain as VR-279. Adapted to grow in continuous cell line NCI-292 (HuT-292)--see Castells et al. Arch Virol. 115:277-288, 1990. After adsorbing virus, feed with medium containing trypsin but NO serum.
References : 32869: Johnson KM, et al. Studies of a new human hemadsorption virus. I. Isolation, properties and characterization. Am. J. Hyg. 71: 81-92, 1960. PubMed: 14407482