ATCC® Number:VR-1457™
Classification: Picornaviridae, Enterovirus, Poliovirus
Agent: Human poliovirus 3 deposited as Poliovirus Type 3
Strain: Mabie Tr (tryptophan-requiring mutant)
Original Source: CNS tissue of human with bulobar poliovyelitis
Depositors: GR Dubes
Biosafety Level:3
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Host Organism : primary rhesus monkey kidney cells primary rhesus monkey kidney cells; primary cynomolgus monkey kidney cells
Incubation : Duration: 1-4 days at 37C
Effect : Yes, in vitro effects: Cytopathic effects with high L-tryptophan; slight or cryptic (stained cells in plaques) with low L-tryptophan
Store at : -70C
Comments : Requires high concentration of L-tryptophan for clear plaques at 37C, pH 7.0 - 7.5 on primary rhesus or cynomolgus monkey cells in tissue culture. Mutates to virus similar to wild type Mabie strain.
References : 33191: Dubes GR, et al. Antigenic variations among type 3 polioviruses. Am. J. Hyg. 70: 91-105, 1959. PubMed: 13670169 33192: Dubes GR, Wenner HA. Virulence of polioviruses in relation to variant characteristics distinguishable on cells in vitro. Virology 4: 275-296, 1957. PubMed: 13496545