ATCC® Number:VR-765™
Classification: Nidovirales, Coronaviridae, Coronavirus,Group 2
Agent: Murine hepatitis virus deposited as Mouse hepatitis virus
Strain: MHV-JHM
Original Source: tissue, animal (brain of 17-day-old Swiss mouse with spontaneous flaccid paralysis of hind legs)
Depositors: J Parker
Biosafety Level:2
Shipped: frozen
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Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 18-48 hours; For best results cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent).
Effect : Yes, in vitro effects: cytopathic effects in tissue culture, fusing and clumping of cells with lots of floating cells
Store at : -70°C (or colder)
References : 24670: et al., Cheever FS. . J. Exp. Med. 90: 181-195, 1949.