ATCC® Number:VR-123™
Classification: Chlamydiaceae
Agent: Chlamydia muridarum Everett et al. deposited as Chlamydia trachomatis (Busacca) Rake
Strain: Mouse Pneumonitis strain Nigg II
Original Source: Normal mice, 1939
Depositors: FB Gordon, J Schachter
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : McCoy cells (ATCCCRL-1696) chicken embryo, yolk sac, mouse, i.n. inoculation
Incubation : Duration: 48 hours at 37.0°C in McCoy cells (ATCCCRL-1696)
Store at : -70.0°C or colder
Comments : Although this agent is not known to be pathogenic for man, it should be handled with care. Mice receiving 1:100 dilution i.n. of infected y.s. occasionally develop pneumonia and die. The Nigg II strain was first deposited by F. B. Gordon as yolk-sac adapted material. It was subsequently redeposited as tissue culture adapted material by J. Schachter. This preparation has tested positive for Mycoplasma contamination. -70.0°C or colder
References : 26511: Pal S, et al. Immunization with an acellular vaccine consisting of the outer membrane complex of Chlamydia trachomatis induces protection against a genital challenge. Infect. Immun. 65: 3361-3369, 1997. PubMed: 9234798 32739: Nigg C. An unidentified virus which produces pneumonia and systemic infection in mice. Science 95: 49, 1942. 32863: Pal S, et al. Intranasal immunization induces long-term protection in mice against a Chlamydia trachomatis genital challenge. Infect. Immun. 64: 5341-5348, 1996. PubMed: 8945586 34375: Kaushic C, et al. Chlamydia trachomatis infection in the female reproductive tract of the rat: influence of progesterone on infectivity and immune response. Infect. Immun. 66: 893-898, 1998. PubMed: 9488372 38940: Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462