Chlamydia suis Everett et al. 病毒拉丁名
(ATCC® VR-1474™) 病毒编号
Classification: Chlamydiaceae /
Product Format 提供形式 : frozen
Classification 分类 Chlamydiaceae Agent Chlamydia suis Everett et al. Strain S45 (porcine isolate)
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions 保藏方法 Vapor phase of liquid nitrogen
Comments 注释 Please see ATCC Virology Guide for more detailed information on propagation
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining
Recommended Host HEp-2 (ATCC® CCL-23™)
Growth Conditions
Temperature: 37℃
Recommendations for Infection: For best results, infection should be performed on an 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells. Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2). Remove cell growth medium and inoculate with disrupted material.
For adsorption, centrifuge at 1500 x g at 25℃ for 1 hour. End adsorption by adding agent growth medium. Incubation: Incubate infected culture for 72 hours at 37℃ in a humidified 5% CO2 atmosphere.
Preservation: Perform slow controlled rate freeze.
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining