ATCC® Number:VR-2033™
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus
Strain: vP-4 [recombinant Vaccinia virus]
Depositors: E Paoletti
Biosafety Level:2
Shipped: frozen
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Host Organism : HeLa cells (ATCCCCL-2); CV-1 cells (ATCCCCL-70)
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : A recombinant virus consisting of an HSV-TK insert in a TK- vaccinia (vTK-79). The recombinant expresses the HSV-TK. Two copies of the herpes virus BamHI fragment coding for TK were cloned (head to tail) into the BamHI site of the vaccinia virus HindIII F-fragment. This construct was cloned into VTK-79 (ATCCVR-2031) by in vivo recombination. [13123]
References : 13123: Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986 13130: Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988 13139: Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520