ATCC® Number:VR-2018™
Classification: Reoviridae, Rotavirus
Agent: Human rotavirus
Strain: Wa (TC adapted)
Original Source: Diarrhea stool from patient positive for rotavirus
Depositors: NIH/NIAID
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-7 days; For best results cells should be 24 to 48 hours old and 100% confluent.
Effect : Yes, in vitro effects: Cytopathic effects, refractile rounding and sloughing
Store at : -70°C (or colder)
Comments : Serotype 1 (G1) for VP7. Trypsin required for in vitro growth. See Wyatt & James. 1982. In vivo and in vitro methods of culture. In: Virus Infections of the Gastrointestinal Tract. H.C. Neu et al.(ed). Marcel Dekker Inc.
References : 3484: James WD, et al. Cultivatable human rotavirus type 2. US Patent 4,341,870 dated Jul 27 1982 4079: ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002). 21463: ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002). 33225: Wyatt RG, et al. Human rotavirus type 2: cultivation in vitro. Science 207: 189-191, 1980. PubMed: 6243190 92344: Water supply -- Domestic type water treatment appliances -- Performance requirements. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4348:1995. 92579: Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02. 92582: Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99. 92587: Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.