ATCC® Number:30634™
Organism: Paramecium tetraurelia Sonneborn
Designations: stock 51 ml pi
Isolation: stock 51mlK, 1954
Depositors: JR Preer
History: ATCC<<--JR Preer<<--T.M. Sonneborn <<--- E. Hanson
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumMax Temperature: 27.0°C Min Temperature: 19.0°C Protocol: ATCCNO: 30300 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, and loosen the cap one half turn. Add 1.0 ml of ATCCmedium 802 bacterized with Enterobacter aerogenes ATCC13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCCmedium 802 in a 20 x 120 mm test tube.
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Comments: Kappa particles have lost R bodies and toxicity
Classification: KINGDOM: Protozoa
References: 4762: Preer LB. Alpha, an infectious macronuclear symbiont of Paramecium aurelia. J. Protozool. 16: 570-578, 1969. PubMed: 5343468 5024: Proc. Natl. Acad. Sci. USA 39: 1228-1233, 1953.