ATCC® Number:50012™
Organism: Paramecium multimicronucleatum Powers and Mitchell
Designations: stock 25
Isolation: lake, Delaware, 1956
Depositors: SL Allen
History: ATCC<<--SL Allen<<--T.M. Sonneborn <<--- L.C. Gilman
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumTemperature: 25.0°C Protocol: ATCCNO: 30300 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, and loosen the cap one half turn. Add 1.0 ml of ATCCmedium 802 bacterized with Enterobacter aerogenes ATCC13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCCmedium 802 in a 20 x 120 mm test tube.
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Comments: syngen 2
Classification: KINGDOM: Protozoa
References: 4803: Allen SL, et al. Intraspecies variability in the esterases and acid phosphatases of Paramecium jenningsi and Paramecium multimicronucleatum: Assignment of unidentified Paramecium: Comparison with the P. aurelia complex. J. Protozool. 30: 155-163, 1983.