ATCC® Number:50058™
Organism: Crypthecodinium cohnii (Seligo) Javornicky
Designations: CHT1
Isolation: Thalassia sp., Coral Harbor, St. John, U.S. Virgin Islands, 1979.
Depositors: CA Beam, M Himes
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 460: A2E6 mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCCmedium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCCmedium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
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Applications: produces docosahexaenoic acids docosahexaenoic acid [5190] [4571]
Comments: Major Sibling Species MI
Classification: KINGDOM: Protozoa
References: 4571: . . Japanese Patent 7,008,221 5124: Seafood products having good taste containing marine microalgae, particularly Crythecodinium cohnii containing docosahexaenoic acid. Japanese Patent 7,008,221 5190: Iizuka T, et al. Marine micro-algae food material containing docosahexaenoic acid, food containing the same and manufacturing method therefor. US Patent 5,547,699 dated Aug 20 1996