ATCC® Number:50392™
Organism: Herpetomonas sp. 2
Designations: McHugh1
Isolation: Culicoides sp. reared from cactus collected in San Antonio, Bexar County, TX, 1993.
Depositors: CP McHugh
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 807: Brain heart infusion blood agarTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: This species is unique since it does not have an emergent flagellum.