ATCC® Number:50651™
Organism: Encephalitozoon intestinalis (Cali et al.) Hartskeerl et al.
Designations: CDC:V297
Isolation: clinical isolate from 26-year-old man with AIDS, California, 1993 [43277]
Depositors: GS Visvesvara
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 722: Minimum essential medium (MEM)Temperature: 35.0°C
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Comments: flow cytometric analysis [38799] detection of drug-induced effects in infected green monkey kidney cells [48251] [47325] Western blot and immunofluorescence analysis [38797] Control strain for enterocytozoan identification [43280] [48252] [43275] [43276] [43278] characterization [43277]
References: 32928: Gatti S, et al. Extraintestinal microsporidiosis in AIDS patients: clinical features and advanced protocols for diagnosis and characterization of the isolates. J. Eukaryot. Microbiol. 44: 79S, 1997. PubMed: 9508459 38797: Croppo GP, et al. Western blot and immunofluorescence analysis of a human isolate of Encephalitozoon cuniculi established in culture from the urine of a patient with AIDS. J. Parasitol. 83: 66-69, 1997. PubMed: 9057698 38799: Moss DM, et al. Flow cytometric analysis of microsporidia belonging to the genus Encephalitozoon. J. Clin. Microbiol. 37: 371-375, 1999. PubMed: 9889221 39769: Moura H, et al. Detection by an immunofluorescence test of Encephalitozoon intestinalis spores in routinely formalin-fixed stool samples stored at room temperature. J. Clin. Microbiol. 37: 2317-2322, 1999. PubMed: 10364604 43275: del Aguila C, et al. Identification of Enterocytozoon bieneusi spores in respiratory samples from an AIDS patient with a 2-year history of intestinal microsporidiosis. J. Clin. Microbiol. 35: 1862-1866, 1997. PubMed: 9196210 43276: Croppo GP, et al. Identification of the microsporidian Encephalitozoon hellem using immunoglobulin G monoclonal antibodies. Arch. Pathol. Lab. Med. 122: 182-186, 1998. PubMed: 9499364 43277: del Aguila C, et al. Ultrastructure, immunofluorescence, western blot, and PCR analysis of eight isolates of Encephalitozoon (Septata) intestinalis established in culture from sputum and urine samples and duodenal aspirates of five patients with AIDS. J. Clin. Microbiol. 36: 1201-1208, 1998. PubMed: 9574677 43278: Bornay-Llinares FJ, et al. Immunologic, microscopic, and molecular evidence of Encephalitozoon intestinalis (Septata intestinalis) infection in mammals other than humans. J. Infect. Dis. 178: 820-826, 1998. PubMed: 9728552 43280: Scaglia M, et al. Asymptomatic respiratory tract microsporidiosis due to Encephalitozoon hellem in three patients with AIDS. Clin. Infect. Dis. 26: 174-176, 1998. PubMed: 9455527 47325: Leitch GJ, et al. Use of fluorescent probe to assess the activities of candidate agents against intracellular forms of Encephalitozoon microsporidia. Antimicrob. Agents Chemother. 41: 337-344, 1997. PubMed: 9021189 48251: He Q, et al. Effects of nifedipine, metronidazole, and nitric oxide donors on spore germination and cell culture infection of the microsporidia Encephalitozoon hellem and Encephalitozoon intestinalis. Antimicrob. Agents Chemother. 40: 179-185, 1996. PubMed: 8787902 48252: Da Silva AJ, et al. Detection of Septata intestinalis (Microsporidia) Cali et al. 1993 using polymerase chain reaction primers targeting the small subunit ribosomal RNA coding region. Mol. Diagn. 2: 47-52, 1997. 70347: del Aguila C, et al. In vitro culture, ultrastructure, antigenic, and molecular characterization of Encephalitozoon cuniculi isolated from urine and sputum samples from a Spanish patient with AIDS. J. Clin. Microbiol. 39: 1105-1108, 2001. PubMed: 11230434 70367: Sobottka I, et al. Disseminated Encephalitozoon (Septata) intestinalis infection in a patient with AIDS: novel diagnostic approaches and autopsy-confirmed parasitological cure following treatment with albendazole. J. Clin. Microbiol. 33: 2948-2952, 1995. PubMed: 8576351