BK polyomavirus 拉丁名
VR-837 编号
BK polyomavirus is propagated in HEL 299 cells (ATCC CCL-137). This strain was isolated in 1970 from the urine of a human kidney transplant patient in London.
Product category
Viruses
Product type
Animal virus
Classification
Polyomaviridae, Polyomavirus
Common name
BK Virus
Isolation source
Urine of a kidney transplant patient.
Geographical isolation
United Kingdom; London
Storage conditions 保藏温度 -70°C or colder
Comments
Hemagglutination of 0.5% GPRBC. Virus MUST be freeze-thaw cycled 6 times for greatest infectivity.
Mycoplasma contamination
Not detectedHost
HEL 299 (ATCC CCL-137)
Effects
cell degeneration; cell rounding; CPE
Complete medium
EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
Temperature 培养温度 37°C
Atmosphere 需氧情况 95% Air, 5% CO2
Incubation
14-23 days, a 5% CO2 in air atmosphere is recommended
Recommendation for infection
For best results, cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent)
Handling notes
Virus MUST be freeze-thaw cycled 6 times for greatest infectivity.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serumVolume
1.0 mL
Deposited as
BK virus
Depositors
G di Mayorca
Type of isolate
Human
Year of origin
1970