Zika virus
VR-1844™
Product categoryViruses
Product typeAnimal virus
ClassificationFlaviviridae, FlavivirusStrain designationFLRIsolation sourceHuman serum.Geographical isolationColombiaApplicationsInfectious disease research
Vector-borne disease research
Zoonotic disease research
Product formatFrozenStorage conditions-70°C or colder
CommentsCPE clearer to observe in VERO C1008 [Vero 76, clone E6, Vero E6] cells (ATCC® CRL-1586™).
Handling information
HostAedes albopictus clone C6/36 (ATCC CRL-1660)
Effectscell sloughing; CPE
Complete mediumEMEM (ATCC® 30-2003™) + 2% FBS (ATCC® 30-2020™)
Temperature28°C
Atmosphere95% Air, 5% CO2
Incubation4-6 days at 28°C in a humidified 5% CO2 atmosphere, until CPE is progressed throughout monolayer.Recommendation for infectionPlate cells 24 hours prior to infection and infect when cultures are 80% confluent. Remove medium and then wash monolayer with PBS or serum free medium prior to inoculation. Inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb for 1 hour at 28°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.Handling notesCPE clearer to observe in VERO C1008 [Vero 76, clone E6, Vero E6] cells (ATCC® CRL-1586™).Key abbreviations°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Quality control specifications
Volume1.0 mL
History
Deposited asZika virusDepositorsR Rico-Hesse, Baylor College of MedicineType of isolateHumanYear of origin2015
Legal disclaimers
Intended useThis product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.WarrantyThe product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect t